Bugs Bunny's first work with Queen Isabella after vacation (8)
I believe the answer is:
bacteria
'bugs' is the definition.
(I've seen this before)
'bunny's first work with queen isabella after vacation' is the wordplay.
'first' indicates taking the first letters.
'work with' becomes 'act' ('act' can be a synonym of 'work'. I am not sure about the 'with' bit.**).
'queen' becomes 'er' (abbreviation for Elizabeth Regina).
'after vacation' says to hollow out the word (remove centre letters) (the inside letters must vacate).
'isabella' with its middle taken out is 'ia'.
The initial letter of 'bunny' is 'b'.
'b'+'act'+'er'+'ia'='BACTERIA'
Can you help me to learn more?
(Other definitions for bacteria that I've seen before include "Single-cell organisms, can be good or bad for you" , "Cabaret I found containing microscopic organisms" , "source of disease" , "Causes of many diseases" , "Microscopic organisms, may carry diseases" .)