Opposed to characters in Constantinople (4)
I believe the answer is:
anti
'opposed to' is the definition.
(I've seen this before)
'characters in constantinople' is the wordplay.
'characters in' indicates a hidden word.
'ANTI' is hidden in the letters of 'constantinople'.
(Other definitions for anti that I've seen before include "Who pooh-poohs all" , "Negative vote" , "Against, informally" , "One who is against (something)" , "Prefix meaning against" .)