Not in favour of worker gaining independence (4)
I believe the answer is:
anti
'not in favour' is the definition.
(I've seen this before)
'worker gaining independence' is the wordplay.
'worker' becomes 'ant' (worker is a type of ant**).
'gaining' means one lot of letters go next to another.
'independence' becomes 'i'.
'ant'+'i'='ANTI'
'of' is the link.
(Other definitions for anti that I've seen before include "Who pooh-poohs all" , "Naysayer" , "Not in favor of" , "Not favouring" , "[NO MAN]" .)