American crossword constructor repulsed by that man, guiding ladies (10)

'guiding ladies' is the definition.
(ushers or usherettes guide people)

'american crossword constructor repulsed by that man' is the wordplay.
'american' becomes 'US' (abbreviation for United States).
'crossword constructor' becomes 'setter' (someone who sets crosswords).
'repulsed' says the letters should be written in reverse.
'by' says to put letters next to each other.
'that man' becomes 'he' ('he' refers to a man).
'setter' in reverse letter order is 'rettes'.
'rettes' put after 'he' is 'herettes'.
'us'+'herettes'='USHERETTES'