Dye making Colin ache terribly (9)
I believe the answer is:
cochineal
'dye' is the definition.
(I know that cochineal is a type of dye)
'colin ache terribly' is the wordplay.
'terribly' indicates an anagram.
'colin'+'ache'='colinache'
'colinache' with letters rearranged gives 'COCHINEAL'.
'making' is the link.
(Other definitions for cochineal that I've seen before include "Red food colouring" , "Crimson dye from crushed insects" , "Red colour" , "Scarlet dye - in a cloche (anag)" , "Scarlet food dye" .)