In spot, one very disturbed piece of turf (5)
I believe the answer is:
divot
'disturbed piece of turf' is the definition.
(piece of turf cut out of the ground by a golf club)
'in spot one very' is the wordplay.
'in' indicates putting letters inside.
'spot' becomes 'dot' (I've seen this before).
'one' becomes 'i' (Roman numeral).
'very' becomes 'v' (abbreviation).
'i'+'v'='iv'
'dot' going around 'iv' is 'DIVOT'.
(Other definitions for divot that I've seen before include "Golfer's dislodged turf" , "Clump of turf for a golfer to replace" , "Loose piece of turf" , "Piece of turf dug out of a lawn" , "Small piece of turf cut out" .)