Lady's out of order unfortunately (5)
I believe the answer is:
sadly
'unfortunately' is the definition.
(I've seen this before)
'lady's out of order' is the wordplay.
'out of order' indicates an anagram.
'ladys' with letters rearranged gives 'SADLY'.
(Other definitions for sadly that I've seen before include "Unhappily" , "Lamentably, unfortunately" , "Alas" , "With sorrow" , "Regrettably" .)