Love the lines, last from hero in play (7)
I believe the answer is:
othello
'play' is the definition.
(play by William Shakespeare)
'love the lines last from hero' is the wordplay.
'love' becomes 'o' (love means zero in tennis).
'lines' becomes 'll' (two abbreviations for line).
'last from' indicates one should take the final letters.
The last letter of 'hero' is 'o'.
'o'+'the'+'ll'+'o'='OTHELLO'
'in' acts as a link.
(Other definitions for othello that I've seen before include "product of 12 [SHAKESPEARE] , inspiring 6 [VERDI]" , "Will's hero" , "Shakespeare's tragic Moor of Venice" , "Shakespeare's general" , "He opposed the Turkish" .)