Queen, possibly, making regular use of craft (3)
I believe the answer is:
cat
'queen possibly' is the definition.
(queen is a kind of cat)
'making regular use of craft' is the wordplay.
'making regular use of' means one should take alternating letters.
The alternating letters of 'craft' are 'CAT'.
(Other definitions for cat that I've seen before include "An old whip with nine tails" , "Feline mammel" , "A tom" , "Tiger, for instance" , "Grimalkin" .)