Temperamental daughter seen in low comedy, ultimately (5)
I believe the answer is:
moody
'temperamental' is the definition.
('moody' can be a synonym of 'temperamental')
'daughter seen in low comedy ultimately' is the wordplay.
'daughter' becomes 'd'.
'seen in' means one lot of letters goes inside another.
'low' becomes 'moo' ('moo' can be a synonym of 'low').
'ultimately' indicates one should take the final letters.
The final letter of 'comedy' is 'y'.
'moo'+'y'='mooy'
'd' placed within 'mooy' is 'MOODY'.
(Other definitions for moody that I've seen before include "Down in the dumps" , "Changeable, sullen" , "Liable to sulking" , "Mercurial" , "Gloomy sullen" .)